Fabrication of 3D alginate scaffold with interconn

Authors: SeHwan Lee A Ra Jo Ghi Pyoung Choi Chang Hee Woo Seung Jae Lee BeomSu Kim HyungKeun You YoungSam Cho

Publish Date: 2013/04/02

Volume: 10, Issue: 2, Pages: 53-59

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Abstract

In this study we fabricated 3D porous scaffold by ‘WireNetwork Molding’ technique with alginate gel which has been used for cartilage regeneration because of the chemical similarity Firstly prepared ETPCSS wires with size of rectangular cross section 600 μm by 600 μm 400 μm by 400 μm respectively and the wires are inserted in designed mold Secondly sterilized 2 wt alginate gel within hMSC human Mesenchymal stem cell was injected into the assembled mold The concentration of hMSC in the used alginate gel is about 5000 cells per scaffold For the gelation of alginate gel the mold was soaked in 5 wt CaCl2 solution for 5 min Subsequently wires are separated from the mold and the mold is removed from alginate gel Consequently the remained alginate scaffold has interconnected pores with a configuration of wirenetwork Additionally to analyze the cellculturing characteristics 1day 3day and 7day cultured scaffolds which encapsulate hMSC are assessed using MTS assay Consequently the optical density of 400 μmWNM scaffolds and 600 μmWNM scaffolds are clearly more increased than control scaffolds without pores

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