Efficacy comparison of nanoniosomal and pegylated

Authors: Mahrokh Babaei Mehdi Ardjmand Azim Akbarzadeh Aliakbar Seyfkordi

Publish Date: 2014/10/02

Volume: 11, Issue: 5, Pages: 350-354

PDF Link

Abstract

In this study Cisplatin was loaded into nanoniosom and pegylated nanoniosom employing reverse phase evaporation method Span 60 cholesterol and Cisplatin were combined together at certain concentrations in this method This study reports the efficacy of nanoniosome Cisplatin and pegylated nanoniosome Cisplatin on brain cancer A172 cell line All of the results are presented from three independent tests The obtained results showed that the stability of prepared formulation was increased by pegylation Encapsulation efficiency and loading efficiency of Cisplatin in pegylated and nonpegylated niosomal formulations were estimated 482±205 436±459 438±028 436±008 respectively The average diameters of pegylated and nonpegylated nanoparticles of niosomes were determined by Zeta sizer which was 2055±460 nm and 2421±510 nm respectively The release of drug was studied by dialysis method The amounts of drug released from pegylated and nonpegylated nanoniosomes were calculated 8273±136 and 9753±055 during 48 hr respectively The toxicity of formulated Cisplatin was studied by MTT assay and the results revealed that the cytotoxicity effect of pegylated nanoniosomal Cisplatin was more than nanoniosomal Cisplatin The IC50 for nanoniosomal Cisplatin was estimated 1232±098 μg/mL while IC50 for pegylated nanoniosomal formulation was calculated 798±114 μg/mL

Keywords: