Effect of type I collagen derived from tilapia sca

Authors: Jia Tang Takashi Saito

Publish Date: 2015/08/16

Volume: 12, Issue: 4, Pages: 231-238

PDF Link

Abstract

Type I collagen is the most abundant extracellular matrix in bone and dentin It was reported to be effective in inducing the osteoblast differentiation Majority of the type I collagen used in scientific research are from bovine skin porcine skin and rat tail Recently a novel type I collagen possessing sound thermal stability was isolated from tilapia fish scale It was reported to be able to induce the differentiation of human mesenchymal stem cells into osteoblasts However no information is available regarding its effect on odontoblastlike cells The purpose of this study was to investigate the effect of type I collagen derived from tilapia fish scale on odontoblastlike cells Type I collagen was immobilized to tissue culture polystyrene dish Biocompatibility of type I collagen was evaluated in terms of initial cell number alkaline phosphatase activity ALP activity odontogenic gene expression and calcific deposition The results showed that type I collagen purified from tilapia fish scale significantly enhanced the cell attachment to nearly twofold as compared to control moreover cells cultured in type I collagenmodified substrate was induced to differentiate toward odontoblast lineage as demonstrated by upregulation of ALP activity on day 7 enhancement of ALP BSP mRNA expression on day 7 and 10 and accelerated mineralization on day 9 It is therefore suggested that type I collagen derived from tilapia scale an underutilized resource holds promise as scaffolding material in the application of tissue engineering in dental field

Keywords: