Growth behavior of endothelial cells according to

Authors: YoungGwang Ko Ju Hee Park Jae Baek Lee Hwan Hee Oh Won Ho Park Donghwan Cho Oh Hyeong Kwon

Publish Date: 2016/08/05

Volume: 13, Issue: 4, Pages: 343-351

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Abstract

Investigating the effect of electrospun fiber diameter on endothelial cell proliferation provides an important guidance for the design of a fabric scaffold In this study we prepared biodegradable polyDLlacticcoglycolic acid PLGA fibrous nonwoven mats with different fiber diameters ranged from 200 nm to 5 µm using the electrospinning technique To control the fiber diameters of PLGA mats 4 mixture solvents hexafluoro2propanol 222trifluoroethanoldimethylformamide 91 222trifluoroethanolhexafluoro2propanol 91 chloroform were used Average diameters were 200 nm 600 nm 15 µm and 50 µm respectively Stereoscopic structure and spatial characterization of fibrous PLGA mats were analyzed using atomic force microscopy and a porosimeter The mechanical properties of PLGA mats were analyzed using a universal testing machine The spreading behavior and infiltration of endothelial cells on PLGA mats were visualized by field emission scanning electron microscopy and hematoxylin and eosin staining Cell proliferation on different PLGA fibers with different diameters was quantified using the MTT assay Cells on 200 nm diameter PLGA mats showed rapid attachment and spreading However the cells did not penetrate the PLGA mat Cells cultured on 600 nm and 15 µm diameter fibers could infiltrate the pores and cell proliferation was dramatically increased after 14 days Secreted prostacyclin from endothelial cells on each mat was measured to examine the ability to inhibit platelet activation This basic study on cell proliferation and fiber diameter with physical characterization provides a foundation for studies examining nonwoven fibrous PLGA mats as a tissue engineering scaffold

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