SMOC1induced osteoblast differentiation involves

Authors: YoungAe Choi DongSung Kim HongIn Shin Eui Kyun Park

Publish Date: 2014/08/07

Volume: 11, Issue: 4, Pages: 304-316

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Abstract

SPARC related modular calcium binding 1 SMOC1 has been implicated in the regulation of the osteoblast differentiation of human bone marrow mesenchymal stem cells BMSCs however it is not clear how it regulates this process In this study we analyzed molecular networks during the SMOC1induced osteoblast differentiation of BMSCs SMOC1 overexpression induced the deposition of minerals and the expression of osteoblast marker genes During osteoblast differentiation induced by SMOC1 overexpression gene profiling was performed using an Affymetrix GeneChip The expression of 191 genes was increased by SMOC1 overexpression and these genes were classified into 17 groups based on gene ontology developmental process 23 cellular component organization 23 cell death 105 cell cycle 99 cell adhesion 79 and cytoskeleton organization 52 Protein interaction analysis also showed the strong interaction of proteins involved in development cell death and cell cycle Consistently proliferation of BMSCs overexpressing SMOC1 was significantly increased compared to BMSCs expressing the control vector These results suggest that SMOC1 induces the osteoblast differentiation of BMSCs by inducing the expression of an array of genes involved in cell growth and development

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