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Title of Journal: Plant Cell Rep

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Abbravation: Plant Cell Reports

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Springer-Verlag

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DOI

10.1007/s10384-011-0042-2

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1432-203X

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Transformation of radish Emphasis Type="Italic"

Authors: ByongJin Park Zaochang Liu Akira Kanno Toshiaki Kameya
Publish Date: 2005/04/21
Volume: 24, Issue: 8, Pages: 494-500
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Abstract

A protocol for producing transgenic radish Raphanus sativus was obtained by using both ultrasonic and vacuum infiltration assisted Agrobacteriummediated transformation The Agrobacterium strain LBA4404 contained the binary vector pBI121LEA late embyogenesis abundant which carried a Group 3 LEA gene from Brassica napus Among six combinations Agrobacteriummediated transformation assisted by a combination of 5min sonication with 5min vacuum infiltration resulted in the highest transformation frequency The existence integration and expression of transferred LEA gene in transgenic T1 plants were confirmed by PCR genomic Southern and Western blot analysis Transgenic radish demonstrated better growth performance than nontransformed control plants under osmotic and salt stress conditions Accumulation of Group 3 LEA protein in the vegetative tissue of transgenic radish conferred increased tolerance to water deficit and salt stress

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