Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Plant Cell Rep

Search In Journal Title:

Abbravation: Plant Cell Reports

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1002/adma.201400410

Search In DOI:

ISSN

1432-203X

Search In ISSN:
Search In Title Of Papers:

Molecular characterization of the Emphasis Type="

Authors: JianZhi Huang TingChi Cheng PeiJung Wen MingHsiun Hsieh FureChyi Chen
Publish Date: 2009/07/28
Volume: 28, Issue: 10, Pages: 1475-1486
PDF Link

Abstract

Two pathways are used by higher plants for the biosynthesis of isoprenoid precursors the mevalonate pathway in the cytosol and a 2Cmethylderythritol 4phosphate MEP pathway in the plastids with 1hydroxy2methyl2Ebutenyl 4diphosphate reductase HDR catalyzing the last step in the MEP pathway In order to understand the contribution of MEP pathway in isoprenoid biosynthesis of Oncidium orchid a fulllength cDNA corresponding to HDR from the flower tissues of Oncidium Gower Ramsey was cloned The deduced OncHDR amino acid sequence contains a plastid signal peptide at the Nterminus and four conserved cysteine residues RTPCR analysis of HDR in Oncidium flowering plants revealed ubiquitous expression in organs and tissues with preferential expression in the floral organs Phylogenetic analysis revealed evolutionary conservation of the encoding HDR protein sequence The genomic sequence of the HDR in Oncidium is similar to that in Arabidopsis grape and rice in structure Successful complementation by OncHDR of an E coli hdr − mutant confirmed its function Transgenic tobacco carrying the OncHDR promoterGUS gene fusion showed expression in most tissues as well as in reproductive organs as revealed by histochemical staining Light induced strong GUS expression driven by the OncHDR promoter in transgenic tobacco seedlings Taken together our data suggest a role for OncHDR as a lightactivated gene

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Characterization of the superoxide dismutase genes of the halophyte Suaeda maritima in Japan and Egypt
  2. Fertile transgenic pearl millet [ Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues
  3. Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli
  4. Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon : the case of pulp carotenoid biosynthesis pathway
  5. Plant regeneration from stem and petal of carnation ( Dianthus caryophyllus L.)
  6. An improved immunofluorescence staining method for chloroplast proteins
  7. Introduction of the tobacco retrotransposon Tto1 into diploid potato
  8. Expression profiles of precursor and mature microRNAs under dehydration and high salinity shock in Populus euphratica
  9. Revelation of ancestral roles of KNOX genes by a functional analysis of Physcomitrella homologues
  10. Isolation and functional analysis of the Catharanthus roseus deacetylvindoline-4- O -acetyltransferase gene promoter
  11. Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum
  12. Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from de novo assembled transcriptome of Catharanthus roseus (L.) G. Don
  13. A rapid and efficient regeneration system for pea ( Pisum sativum ), suitable for transformation
  14. Engineering low phorbol ester Jatropha curcas seed by intercepting casbene biosynthesis
  15. Production of marker-free transgenic rice expressing tissue-specific Bt gene
  16. Production of marker-free transgenic rice expressing tissue-specific Bt gene
  17. Mammalian pro-apoptotic bax gene enhances tobacco resistance to pathogens
  18. Molecular control of stem cell maintenance in shoot apical meristem
  19. Gene identification in black cohosh ( Actaea racemosa L.): expressed sequence tag profiling and genetic screening yields candidate genes for production of bioactive secondary metabolites
  20. Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape ( Brassica napus L.) genotypes differing in responsiveness to androgenesis induction
  21. The phytohormone crosstalk paradigm takes center stage in understanding how plants respond to abiotic stresses
  22. Plant regeneration and stable transformation in the floricultural plant Cleome spinosa , a C 3 plant closely related to the C 4 plant C. gynandra
  23. Exogenous application of salicylic acid alleviates cadmium toxicity and reduces hydrogen peroxide accumulation in root apoplasts of Phaseolus aureus and Vicia sativa
  24. Cloning and functions analysis of a pyruvate dehydrogenase kinase in Brassica napus
  25. Six Medicago truncatula Dicer-like protein genes are expressed in plant cells and upregulated in nodules
  26. Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance
  27. Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance
  28. Genetic transformation of Leymus chinensis with the PAT gene through microprojectile bombardment to improve resistance to the herbicide Basta
  29. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines
  30. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines
  31. Root proteomics reveals cucumber 24-epibrassinolide responses under Ca(NO 3 ) 2 stress
  32. Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols
  33. High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR
  34. Agrobacterium -mediated co-transformation of rice using two selectable marker genes derived from rice genome components
  35. In vitro hermaphrodism induction in date palm female flower
  36. Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize ( Zea mays L.)
  37. Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize ( Zea mays L.)
  38. A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots
  39. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants
  40. Transformation of radish ( Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus
  41. Proteome analysis of embryogenic cell suspensions of cowpea ( Vigna unguiculata )
  42. Caspase-resistant VirD2 protein provides enhanced gene delivery and expression in plants
Search Result: