Authors: Yurong Qin Zhongxin Duan Xinli Xia Weilun Yin
Publish Date: 2011/06/25
Volume: 30, Issue: 10, Pages: 1893-
Abstract
MicroRNAs miRNAs are small noncoding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs Populus euphratica is a typical stressresistant sessile organism that grows in desert areas Here we identified sequences of 12 miRNA precursors from 11 families and 13 mature miRNAs from 12 families by PCR amplification in P euphratica To detect expression differences in mature miRNAs and their precursors under dehydration and high salinity shock in P euphratica we examined 14 miRNA precursors from 13 miRNA families and 17 mature miRNAs from 17 miRNA families using the SYBR Green RT–PCR assay This is the first report of expression profiles for both precursor and mature miRNAs in P euphratica By profiling both the mature miRNAs and the precursors under abiotic stress shock it was possible to identify miRNA whose processing is regulated during stress shock environments A majority of the genes predicted to be targets for plant miRNAs are involved in development stress resistance and metabolic processes We have cloned and experimentally identified in vivo five of the predicted target genes and quantified the five target mRNAs from the same RNA sample simultaneously Based on this study we propose some regulatory pathways that illustrate the important role that miRNAs play in response to abiotic stress shock in P euphraticaThis work was supported by the “948” Project of the State Forestry Administration of China 2007401 the National Natural Science Foundation of China No 30730077 30972339 31070597 the Program for New Century Excellent Talents in University of China NCET070083 and National Key Technologies RD Program of China 2011BAD38B01
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