Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Plant Cell Rep

Search In Journal Title:

Abbravation: Plant Cell Reports

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1002/ardp.18802160523

Search In DOI:

ISSN

1432-203X

Search In ISSN:
Search In Title Of Papers:

Expression profiles of precursor and mature microR

Authors: Yurong Qin Zhongxin Duan Xinli Xia Weilun Yin
Publish Date: 2011/06/25
Volume: 30, Issue: 10, Pages: 1893-
PDF Link

Abstract

MicroRNAs miRNAs are small noncoding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs Populus euphratica is a typical stressresistant sessile organism that grows in desert areas Here we identified sequences of 12 miRNA precursors from 11 families and 13 mature miRNAs from 12 families by PCR amplification in P euphratica To detect expression differences in mature miRNAs and their precursors under dehydration and high salinity shock in P euphratica we examined 14 miRNA precursors from 13 miRNA families and 17 mature miRNAs from 17 miRNA families using the SYBR Green RT–PCR assay This is the first report of expression profiles for both precursor and mature miRNAs in P euphratica By profiling both the mature miRNAs and the precursors under abiotic stress shock it was possible to identify miRNA whose processing is regulated during stress shock environments A majority of the genes predicted to be targets for plant miRNAs are involved in development stress resistance and metabolic processes We have cloned and experimentally identified in vivo five of the predicted target genes and quantified the five target mRNAs from the same RNA sample simultaneously Based on this study we propose some regulatory pathways that illustrate the important role that miRNAs play in response to abiotic stress shock in P euphraticaThis work was supported by the “948” Project of the State Forestry Administration of China 2007401 the National Natural Science Foundation of China No 30730077 30972339 31070597 the Program for New Century Excellent Talents in University of China NCET070083 and National Key Technologies RD Program of China 2011BAD38B01


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Characterization of the superoxide dismutase genes of the halophyte Suaeda maritima in Japan and Egypt
  2. Fertile transgenic pearl millet [ Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues
  3. Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli
  4. Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon : the case of pulp carotenoid biosynthesis pathway
  5. Plant regeneration from stem and petal of carnation ( Dianthus caryophyllus L.)
  6. Molecular characterization of the Oncidium orchid HDR gene encoding 1-hydroxy-2-methyl-2-( E )-butenyl 4-diphosphate reductase, the last step of the methylerythritol phosphate pathway
  7. An improved immunofluorescence staining method for chloroplast proteins
  8. Introduction of the tobacco retrotransposon Tto1 into diploid potato
  9. Revelation of ancestral roles of KNOX genes by a functional analysis of Physcomitrella homologues
  10. Isolation and functional analysis of the Catharanthus roseus deacetylvindoline-4- O -acetyltransferase gene promoter
  11. Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum
  12. Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from de novo assembled transcriptome of Catharanthus roseus (L.) G. Don
  13. A rapid and efficient regeneration system for pea ( Pisum sativum ), suitable for transformation
  14. Engineering low phorbol ester Jatropha curcas seed by intercepting casbene biosynthesis
  15. Production of marker-free transgenic rice expressing tissue-specific Bt gene
  16. Production of marker-free transgenic rice expressing tissue-specific Bt gene
  17. Mammalian pro-apoptotic bax gene enhances tobacco resistance to pathogens
  18. Molecular control of stem cell maintenance in shoot apical meristem
  19. Gene identification in black cohosh ( Actaea racemosa L.): expressed sequence tag profiling and genetic screening yields candidate genes for production of bioactive secondary metabolites
  20. Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape ( Brassica napus L.) genotypes differing in responsiveness to androgenesis induction
  21. The phytohormone crosstalk paradigm takes center stage in understanding how plants respond to abiotic stresses
  22. Plant regeneration and stable transformation in the floricultural plant Cleome spinosa , a C 3 plant closely related to the C 4 plant C. gynandra
  23. Exogenous application of salicylic acid alleviates cadmium toxicity and reduces hydrogen peroxide accumulation in root apoplasts of Phaseolus aureus and Vicia sativa
  24. Cloning and functions analysis of a pyruvate dehydrogenase kinase in Brassica napus
  25. Six Medicago truncatula Dicer-like protein genes are expressed in plant cells and upregulated in nodules
  26. Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance
  27. Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance
  28. Genetic transformation of Leymus chinensis with the PAT gene through microprojectile bombardment to improve resistance to the herbicide Basta
  29. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines
  30. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines
  31. Root proteomics reveals cucumber 24-epibrassinolide responses under Ca(NO 3 ) 2 stress
  32. Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols
  33. High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR
  34. Agrobacterium -mediated co-transformation of rice using two selectable marker genes derived from rice genome components
  35. In vitro hermaphrodism induction in date palm female flower
  36. Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize ( Zea mays L.)
  37. Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize ( Zea mays L.)
  38. A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots
  39. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants
  40. Transformation of radish ( Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus
  41. Proteome analysis of embryogenic cell suspensions of cowpea ( Vigna unguiculata )
  42. Caspase-resistant VirD2 protein provides enhanced gene delivery and expression in plants

Search Result: