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Title of Journal: Plant Cell Rep

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Abbravation: Plant Cell Reports

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Springer-Verlag

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10.1007/978-94-007-2460-0_8

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1432-203X

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Sequence stability of the TDNA – plant junctions

Authors: Nina Papazova Pieter Windels Ann Depicker Isabel Taverniers Isabel RoldanRuiz Anne Milcamps Erik Van Bockstaele Guy Van Den Eede Marc De Loose
Publish Date: 2006/06/30
Volume: 25, Issue: 12, Pages: 1362-1368
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Abstract

The stability of the inserted transgenes and particularly the junction regions of transgenic events is a concern of food labeling traceability and post release monitoring as these regions are used for development of eventspecific DNAbased detection methods During the standard agricultural breeding practices the transgenic lines can be exposed to completely different conditions than those in the laboratory environment Some of these conditions have the potential to affect the stability of the transgenic locus and the surrounding DNA As tissue culture is recognized as a stressful and mutagenic factor we have analyzed the effect of this process on the stability of the junction regions at nucleotide level in five Arabidopsis thaliana transgenic lines in comparison with the respective integration loci in ColO and C24 ecotypes No indication of any kind of alteration at nucleotide level of the junctions was found The relevance of the stability of the plant–TDNA junction regions for application of the DNAbased methods in commercial transgenic plants is discussedThis work is performed in the frame of project “Development of methods for the detection identification and quantification of genetically modified organisms” contact no 18687200111 F1ED ISP BE funded by ECJRC We thank Elien Glorieus Kristien Janssens and Cindy Merckaert for their excellent experimental work

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