Authors: Yahong An Heng Zhou Min Zhong Jin Sun Sheng Shu Qiaosai Shao Shirong Guo
Publish Date: 2016/03/01
Volume: 35, Issue: 5, Pages: 1081-1101
Abstract
NO3 − and Ca2+ are the main anion and cation of soil secondary salinization during greenhouse cultivation Brassinosteroids BRs steroidal phytohormones regulate various important physiological and developmental processes and are used against abiotic stress A twodimensional electrophoresis gel coupled with MALDITOF/TOF MS was performed to investigate the effects of exogenous 24epibrassinolide EBL on proteomic changes in cucumber seedling roots under CaNO32 stress A total of 80 differentially accumulated protein spots in response to stress and/or exogenous EBL were identified and grouped into different categories of biological processes according to Gene Ontology Under CaNO32 stress proteins related to nitrogen metabolism and lignin biosynthesis were induced while those related to cytoskeleton organization and cellwall neutral sugar metabolism were inhibited However the accumulation of abundant proteins involved in protein modification and degradation defence mechanisms against antioxidation and detoxification and lignin biosynthesis by exogenous EBL might play important roles in salt tolerance Realtime quantitative PCR was performed to investigate BR signalling BR signalling was induced intracellularly under CaNO32 stress Exogenous EBL can alleviate the root indices effectively reduce the Ca2+ content and increase the K+ content in cucumber roots under CaNO32 stress This study revealed the differentially expressed proteins and BR signallingassociated mRNAs induced by EBL in cucumber seedling roots under CaNO32 stress providing a better understanding of EBLinduced salt resistance in cucumber seedlings The mechanism for alleviation provides valuable insight into improving CaNO32 stress tolerance of other horticultural plants
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