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Title of Journal: Plant Cell Rep

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Abbravation: Plant Cell Reports

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Springer Berlin Heidelberg

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DOI

10.1002/maco.200790077

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1432-203X

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Endogenous ABA concentration and cytoplasmic membr

Authors: Ewa Dubas Franciszek Janowiak Monika Krzewska Tomasz Hura Iwona Żur
Publish Date: 2013/06/05
Volume: 32, Issue: 9, Pages: 1465-1475
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Abstract

Changes in plasma membrane fluidity MF and ABA content associated with androgenesis induction were under the study Both parameters were monitored in microspores of two Brassica napus L genotypes differing in their response to androgenic induction under heat 1 day at 32 °C MF was assessed by DPH method ABA content was evaluated by ELISA Heat caused microspores’ plasma membrane to become more rigid Lower MF in microspores of ‘DH 4079’ of high androgenic potential seems to maintain proper cell protection and leads to efficient embryogenesis induction Plasma membrane remodelling coincided with changes of ABA content in microspores and in the culture medium in both genotypes ABA concentration μM and ABA content fmol per 104 microspores or pmol g−1 FW were for the first time measured in microspores ABA concentration μM in microspores and in the culture medium nM differed significantly for the genotype and the treatment The interaction between both variables was also significant In general ABA content ranged from 35 to 871 fmol per 104 microspores The highest content of ABA was detected in ‘DH 4079’ microspores at 32 °C Assuming a mean microspores’ radius of 10 μm it corresponds to ABA concentration of 21 μM Heat shock resulted in quantum of medium pH reduction 01–02 and increased levels of ABA in microspores and in the medium of both tested genotypes However heat induced increase of ABA content in microspores of nonresponsive ‘Campino’ had no clearcut impact on androgenesis induction efficiency which suggests a more complex mechanism of process initiation

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