Authors: Quan Wang Fang Yuan Qifang Pan Meiya Li Guofeng Wang Jingya Zhao Kexuan Tang
Publish Date: 2009/12/25
Volume: 29, Issue: 2, Pages: 185-192
Abstract
Madagascar periwinkle Catharanthus roseus produces many therapeutically valuable terpenoid indole alkaloids TIAs such as vinblastine and vincristine derived from the monomers vindoline and catharanthine Deacetylvindoline4Oacetyltransferase DAT is a key enzyme for the terminal step of vindoline biosynthesis In this research the DAT gene promoter was cloned sequenced and analyzed An approximately 1773 bp genomic DNA fragment of DAT promoter was obtained Sequence analysis revealed that DAT promoter contained several potential regulatory elements which were involved in the regulation of gene expression To investigate its function the promoter fragments with 5′ deletions and gainoffunction deletions were fused to GUS reporter gene and their expressions were analyzed by transient expression in C roseus cell suspensions The regulatory activity of DAT promoter was identified with fluorescence quantitative assays Three TGACG motifs and one inverted motif CGTCA between −808 and −1086 bp in the DAT promoter were found to be involved in methyljasmonate signal transduction pathwayThe authors thank Dr Lingjian Wang from Shanghai Institute of Plant Physiology and Ecology for the help with GUS activity assay This work was supported by China National HighTech “863” Program 2007AA10Z189 “973” Program 2007CB108805 China Transgenic Research Program 2008ZX08002001 Shanghai Science and Technology Committee 08391911800 and Shanghai Leading Academic Discipline Project B209
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