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Title of Journal: Plant Cell Rep

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Abbravation: Plant Cell Reports

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Springer-Verlag

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1432-203X

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Aminoglycoside antibiotics structure functions a

Authors: I M G Padilla L Burgos
Publish Date: 2010/07/20
Volume: 29, Issue: 11, Pages: 1203-1213
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Abstract

Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material The bacterial gene nptII coding for a neomycin phosphotransferase and the hpt gene coding for a hygromycin phosphotransferase are frequently used These enzymes detoxify aminoglycoside antibiotics by phosphorylation thereby permitting cell growth in the presence of antibiotics Nevertheless the screening for transgenic regenerated shoots is often partial and difficult due to regeneration of escapes and chimeras These difficulties can be caused in part by an incorrect assumption about the mode of action of antibiotics in bacterial and eukaryotic cells and in in vitro tissue culture The information contained in this review could be useful to establish better selection strategies by taking into account factors such as explant complexity transformation and selection protocols that allow better accessibility to cells of Agrobacterium and antibiotics and faster regeneration methods that avoid collateral effects of antibiotics on recovered putative transgenic shootsThis work was partially supported by BIOCARM BIOAGR07/040011 and Séneca 08665/PI/08 IMGP thanks the Spanish Council of Scientific Research CSIC for a I3P postdoctoral contract The authors thank Prof Fernando PliegoAlfaro for the critical review of the manuscript and Dr David J Walker for English language editing

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