Authors: Maria Angélica Oliveira Raymond Goulder
Publish Date: 2006/04/27
Volume: 568, Issue: 1, Pages: 29-42
Abstract
The responses of epilithic bacterial and algal communities to sewagetreatmentworks STW effluent were studied in three streams in North Yorkshire England using both conventional microbiological techniques and the techniques of molecular genomics Cod Beck Thornton Beck and the River Wiske were visited in May–June 2000 and January 2001 and the epilithic communities on submerged stones were sampled to determine chlorophylla leucine assimilation bacterial abundance identity and abundance of microalgae and epilithon dry and organic weights Additionally DNA from the epilithon of stones collected in March–April and November 2000 was extracted and samples were amplified using universal primers appropriate respectively for bacteria Muyzer et al 1993 Applied and Environmental Microbiology 59 695–700 and microscopic phototrophs Nübel et al 1997 Applied and Environmental Microbiology 63 3327–3332 followed by DGGE Denaturing Gradient Gel Electrophoresis Differences in algal community composition and abundance were greater between streams than between sites upstream and downstream of the STW outfalls DGGE banding profiles of bacterial communities revealed grouping according to stream and not the formation of communities characteristic of downstream sites seasonal variation was also evident Thus the discharge of STW effluent to the streams did not bring about the development of communities that are characteristically associated with organic pollution Changes in composition and structure of bacterial and microalgal epilithic communities were detected but the relatively high quality effluents had only mild effects not altering the environments sufficiently to override the natural differences between the three streams
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