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Title of Journal: Hydrobiologia

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Abbravation: Hydrobiologia

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Springer Netherlands

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DOI

10.1016/0037-7856(77)90166-4

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1573-5117

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Extensive analysis of nuclear cistron rDNA sequenc

Authors: JangSeu Ki Heum Gi Park JaeSeong Lee
Publish Date: 2010/11/30
Volume: 666, Issue: 1, Pages: 3-9
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Abstract

As a special reference of the nuclear cistron rDNA of cyclopoid copepods we obtained the entire DNA sequence of a single rDNA repeat unit from Paracyclopina nana Cyclopoida Cyclopettidae The genomic organization of P nana rDNA 7974 bp 517 of GC was observed to be 18S–ITS1–58S–ITS2–28S–IGS in order Comparative analyses of the rDNA between P nana and other copepods showed that the intergenic spacer IGS was highly informative and divergent while other coding regions are relatively conserved We detected eleven polyT tracts in the IGS demonstrating the high AT content in this region In addition many subrepeat sequence patterns eg AG AT GC CCG TC such as microsatellites were identified from the rDNA IGS of P nana In this article we show the first complete sequence of rDNA from the order Cyclopoida providing a better understanding of molecular characteristics in molecular taxonomyWe thank Mr Winson K Chan for his comments on the manuscript This work was supported by a grant of Korea Research Foundation funded to Huem Gi Park KRF2006C00699 JangSeu Ki was a recipient of a postdoctoral fellowship funded by the Korea Research Foundation Grant KRF2007355C00059

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