Authors: JangSeu Ki IlChan Kim JaeSeong Lee
Publish Date: 2008/10/21
Volume: 616, Issue: 1, Pages: 229-239
Abstract
Nuclear ribosomal DNAs rDNA constitute a multigene family with tandemly arranged units linked by an intergenic spacer IGS Here we present the complete DNA sequence 7731 bp of a single repeat unit of an rDNA sequence from the moon jelly Aurelia sp1 Cnidaria Scypozoa The tandemly repeated rDNA units consisted of coding and noncoding regions whose arrangement was 18S rDNA 1814 bp 462 of GC contentinternal transcribed spacer 1 ITS1 272 bp 39758S rDNA 158 bp 507ITS2 278 bp 51428S rDNA 3606 bp 497IGS 1603 bp 456 GC composition in the single unit of rDNA was 478 None of the 5S rDNA was found in the repeat units Putative structures of a termination transcription signal polyT tract and promoterlike birepeats within the noncoding region were also identified A block of minisatellites with five repeats was detected within the IGS Comparative analyses of parsimony and dot plots showed that the IGS was highly informative The sequence revealed here was the first completion of rDNA from the phylum Cnidaria using as a model of rDNA for making molecular comparisons of jellyfish membersWe would like to thank Drs Sheikh Raisuddin and HansUwe Dahms for English editing on the manuscript We are very grateful to Dr Timothy J Page and two anonymous reviewers for reading and critical comments This work was supported by grants of KOSEF NRL 2006 and ETEP 2006 funded to JaeSeong Lee and by a grant of KOSEF 2007 funded to Heum Gi Park Thus work was also supported by a grant from Korea Polar Research Institute PE08050 funded to IlChan Kim
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