Authors: Shuai Ma Bingyin Han Volker A R Huss Xiaowen Hu Xuepiao Sun Jiaming Zhang
Publish Date: 2015/05/13
Volume: 760, Issue: 1, Pages: 81-89
Abstract
Open rooftop incubators may provide a costeffective way for producing microalgal biomass for biodiesel production without requiring large land areas While cultivating a Chlorella vulgaris strain with high oil content in a rooftop incubator we identified an invading thermoresistant green alga which replaced the original culture The isolated strain HTA165 had the typical Chlorella morphology with spherical cells and a cupshaped parietal chloroplast The protoplast contained a single pyrenoid traversed by a doublelayered thylakoid and enveloped by a single starch sheath The oil content of this strain was only 215 one third of the original strain This strain grew at 42 °C with an optimum of 33 °C Heat treatment of 45 °C for 3 h per day did not decrease its growth rate suggesting that it has a high potential in biotechnological applications Phylogenetic analyses revealed that HTA165 belongs to the genus Chlorella but a specific relationship to any species was not indicated by nuclear and chloroplast small subunit rRNA genes However based on at least one compensating base change CBC and a few hemiCBCs in the ITS2 secondary structure of closely related species we describe strain HTA165 as a novel thermotolerant Chlorella species C thermophilaThis work was supported by Hainan Provincial Science and Technology Key Program GCZX2013001 ZDZX2013023 and International Science and Technology Cooperation Program of China 2014DFA30680 and Natural Science Foundation of Hainan Province 309051
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