Authors: Pelin Kelicen Agneta Nordberg
Publish Date: 2006/05/06
Volume: 26, Issue: 3, Pages: 225-235
Abstract
1 The analogies between the processing of amyloid precursor protein APP and other transmembrane sterol regulatory element binding proteins SREBPs inspired us to conduct further studies on whether βamyloid Aβ affects aromatase by interacting with APP and SREBP3 Cellular extracts were subjected to immunoblot analysis using antiAPP antiaromatase and antiSREBP1 In these cell lines we detected aromatase 55 kDa SREBP cleavage product 68 kDa and APP precursor 100–95 kDa and cleavage product 60 kDa by immunoblotting Aromatase and SREBP levels were elevated in the cells incubated 24 h in experimental media and were attenuated in Aβsupplemented experimental media4 The disturbance of cholesterol homeostasis appears to be an important factor in the pathogenesis of Alzheimers disease These findings may have important implications for understanding the mechanisms of the aromatase enzyme gene in disease states such as Alzheimer’sWe thank Dr Xiao Zhang for his valuable consultation and provision of materials This work was supported in part by grants from the Swedish Medical Research Council project no 05817 Stiftelsen för Gamla Tjänarinnor Stohnes Foundation and Swedish Alzheimer Foundation
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