Olfactory Ensheathing CellConditioned Medium Reve

Authors: QingQing Fu Li Wei Javier Sierra JianZhang Cheng María Teresa MorenoFlores Hua You HuaRong Yu

Publish Date: 2016/11/02

Volume: 37, Issue: 6, Pages: 1043-1054

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Abstract

Olfactory ensheathing cells OECs are a type of glia from the mammalian olfactory system with neuroprotective and regenerative properties βAmyloid peptides are a major component of the senile plaques characteristic of the Alzheimer brain The amyloid beta Aβ precursor protein is cleaved to amyloid peptides and Aβ25–35 is regarded to be the functional domain of Aβ responsible for its neurotoxic properties It has been reported that Aβ25–35 triggers reactive oxygen species ROSmediated oxidative damage altering the structure and function of mitochondria leading to the activation of the mitochondrial intrinsic apoptotic pathway Our goal is to investigate the effects of OECs on the toxicity of aggregated Aβ25–35 in human neuroblastoma SHSY5Y cells For such purpose SHSY5Y cells were incubated with Aβ25–35 and OECconditioned medium OECCM OECCM promoted the cell viability and reduced the apoptosis and decreased the intracellular ROS and the lipid peroxidation In the presence of OECCM mRNA and protein levels of antioxidant enzymes SOD1 and SOD2 were upregulated Concomitantly OECCM decreased mRNA and the protein expression levels of cytochrome c caspase9 caspase3 and Bax in SHSY5Y cells and increased mRNA and the protein expression level of Bcl2 However OECCM did not alter intracellular Ca2+ concentration in SHSY5Y cells Taken together our data suggest that OECCM ameliorates Aβ25–35induced oxidative damage in neuroblastoma SHSY5Y cells by inhibiting the mitochondrial intrinsic pathway These data provide new insights into the functional actions of OECCM on oxidative stressinduced cell damageThis work was supported by grants from the National Natural Science Foundation of China 81670180 81370077 and 81001220 the cuttingedge research project of the Chongqing Science and Technology Committee CSTC2014JCYJA10014 the Scientific and Technological Research Programme of Chongqing Municipal Education Commission KJ130320 NIG Collaborative Research Program 2016A24 the Chongqing Science and Technology Committee CSTC2016JCYJA0083 Beijing Municipal Science and Technology Commission and Chongqing Municipal Commission of Health and Family Planning 2016MSXM103 The funders had no role in the study design data collection and analysis decision to publish or preparation of the manuscript

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