Investigation of Long Noncoding RNA Expression Pr

Authors: Yaohui Ni Hua Huang Yaqin Chen Maohong Cao Hongzhi Zhou Yuanyuan Zhang

Publish Date: 2016/05/05

Volume: 37, Issue: 2, Pages: 329-338

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Abstract

Genetics is considered as an important risk factor in the pathological changes of Parkinson’s disease PD Substantia nigra SN is thought to be the most vulnerable area in this process In recent decades however few related long noncoding RNAs lncRNAs in the SN of PD patients had been identified and the functions of those lncRNAs had been studied even less In this study we sought to investigate the lncRNA expression profiles and their potential functions in the SN of PD patients We screened lncRNA expression profiles in the SN of PD patients using the lncRNA mining approach from the ArrayExpress database which included GSE20295 The samples were from 11 of PD and 14 of normal tissue samples We identified 87 lncRNAs that were altered significantly in the SN during the occurrence of PD Among these lncRNAs lncRNA AL049437 and lncRNA AK021630 varied most dramatically AL049437 was upregulated in the PD samples while AK021630 was downregulated Based on the results we focused on the potential roles of the two lncRNAs in the pathogenesis of PD by the knockdown of the expression of AL049437 or AK021630 in human neuroblastoma SHSY5Y cell line Results indicated that the reduction in AL049437 level increased cell viability mitochondrial transmembrane potential Δψm mitochondrial mass and tyrosine hydroxylase TyrH secretion By contrast the knockdown of AK021630 resulted in the opposite effect Based on these results we speculated that lncRNA AL049437 likely contributed to the risk of PD while lncRNA AK021630 likely inhibited the occurrence of PD

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