Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Publisher
Springer-Verlag
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Authors: D Koma H Yamanaka K Moriyoshi T Ohmoto K Sakai
Publish Date: 2007/07/27
Volume: 11, Issue: 6, Pages: 769-779
Abstract
A novel extracellular serine protease derived from Thermoanaerobacter tengcongensis designated tengconlysin was successfully overexpressed in Escherichia coli as a soluble protein by recombination of an Nterminal Pel B leader sequence instead of the original presequence and Cterminal 6× histidine tags The purified protein was activated by 01 sodium dodecyl sulfate SDS treatment but not by thermal treatment The molecular weight of tengconlysin estimated by SDSpolyacrylamide gel electrophoresis analysis and gel filtration chromatography was 379 and 362 kDa respectively suggesting that the enzyme is monomeric The Nterminal sequence of mature tengconlysin was LDTAT suggesting that it is a preproprotein containing a 29 amino acid presequence predicted from the SigP program and a 117 amino acid prosequence in the Nterminus The Cterminal putative propeptide position 469–540 in the preproprotein did not inhibit the protease activity The optimum temperature for tengconlysin activity was 90°C in the presence of 1 mM calcium ions and the optimum pH ranged from 65 to 70 Activity inhibition studies suggest that the protease is a serine protease The protease was stable in 01 SDS and 1–4 M urea at 70°C in the presence of calcium ions and was activated by the denaturing agents
Keywords:
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- Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
- The amino acid composition of proteins from anaerobic halophilic bacteria of the order Halanaerobiales
- Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6
- The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii
- Structural and functional characterization of aspartate racemase from the acidothermophilic archaeon Picrophilus torridus
- Novel ultramicrobacterial isolates from a deep Greenland ice core represent a proposed new species, Chryseobacterium greenlandense sp. nov.
- Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity
- Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius
- Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A
- The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
- Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world
- Archaeal diversity: temporal variation in the arsenic-rich creek sediments of Carnoulès Mine, France
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon
- Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan
- Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
- Identification of replication origins in the genome of the methanogenic archaeon, Methanocaldococcus jannaschii
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)
- Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia
- Identification of iron-reducing Thermus strains as Thermus scotoductus
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
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