Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: Nadia Khelifi Emna Ben Romdhane Abdeljabbar Hedi Anne Postec MarieLaure Fardeau Moktar Hamdi JeanLuc Tholozan Bernard Ollivier Agnès HirschlerRéa
Publish Date: 2010/04/01
Volume: 14, Issue: 3, Pages: 297-304
Abstract
A novel thermophilic anaerobic and microaerophilic bacterium optimal growth in the presence of 5–10 O2 strain Nad S1T was isolated from the terrestrial hot spring of Hammam Sidi Jdidi Nabeul Tunisia Cells were motile rods having a Grampositive cell wall structure Strain Nad S1T grew optimally at 55°C range 37–70°C Optimum pH for growth was 65–70 It was halotolerant growing with NaCl up to 7 optimum concentration 15–30 It grew chemoorganotrophically on various carbohydrates organicacids and aminoacids as energy sources or chemolithotrophically on H2 using nitrate as terminal electron acceptor Beside oxygen under microaerobic conditions and nitrate nitrite was also used Nitrate was completely reduced to N2 No fermentation occurred The genomic DNA G + C content was 418 mol Based on 16S rRNA gene sequence analysis strain Nad S1T belongs to the Bacillaceae family within the class ‘Bacilli’ Because of its phylogenetic and phenotypic characteristics we propose this isolate to be assigned as a novel genus and a novel species within the domain Bacteria Microaerobacter geothermalis gen nov sp nov The type strain is Nad S1T =DSM 22679T =JCM 16213TWe thank Manon Joseph for doing electron microscopy Professor Jean Paul Euzéby for assistance with the naming of the organism and Dr Pierre Roger for revising the manuscript This work was supported in part by a French CNRSINSU grant BIOHYDEX EC2COMicrobiEn project
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