Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: Xueguo Song Qihong Huang Jinfeng Ni Yang Yu Yulong Shen
Publish Date: 2016/06/11
Volume: 20, Issue: 4, Pages: 537-546
Abstract
DExD/Hbox helicases represent the largest family of helicases They belong to superfamily 2 helicases and participate in nucleotide metabolism ribosome biogenesis and nucleocytoplasmic transport The biochemical properties and structures of some DExD/Hbox helicases in the archaea have been documented but many of them have not been characterized and reports on in vivo functional analyses are limited In this study we attempted gene knockout of 8 putative DExD/Hbox helicases in Sulfolobus islandicus REY15A and obtained two deletion mutants SiRe 0681 and SiRe 1605 We determined that ΔSiRe 0681 grew faster than wild type cells in the presence of methyl methanesulfonate MMS Flow cytometry analysis showed that this strain had fewer G1/S phase cells than the wild type and the genes coding for cell division proteins were upregulated The stain ΔSiRe 1605 was more sensitive to MMS than the wild type cell and many nucleotide metabolism and DNA repair enzymes were found to be downregulated Intriguingly deletion of either gene led to silencing simultaneously of over 80 genes located at a specific region This study provides a novel insight into the in vivo functions of predicted DExD/Hbox family helicases in the archaeaWe thank Dr Qunxin She from University of Copenhagen for providing S islandicus strains and the plasmid and all the members of our lab for helpful discussions This work was supported by grants from the National Science Foundation of China 31470046 to YS and 31470200 to JN
Keywords:
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Other Papers In This Journal:
- Bio-removal of cadmium by growing deep-sea bacterium Pseudoalteromonas sp. SCSE709-6
- Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
- The amino acid composition of proteins from anaerobic halophilic bacteria of the order Halanaerobiales
- Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6
- The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii
- Structural and functional characterization of aspartate racemase from the acidothermophilic archaeon Picrophilus torridus
- Novel ultramicrobacterial isolates from a deep Greenland ice core represent a proposed new species, Chryseobacterium greenlandense sp. nov.
- Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity
- Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius
- Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A
- The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
- Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world
- Archaeal diversity: temporal variation in the arsenic-rich creek sediments of Carnoulès Mine, France
- Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon
- Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan
- Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
- Identification of replication origins in the genome of the methanogenic archaeon, Methanocaldococcus jannaschii
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)
- Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia
- Identification of iron-reducing Thermus strains as Thermus scotoductus
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
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