Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: Kento Takahashi Fumika Nakanishi Takeo Tomita Nagisa Akiyama Kerstin Lassak SonjaVerena Albers Tomohisa Kuzuyama Makoto Nishiyama
Publish Date: 2016/09/02
Volume: 20, Issue: 6, Pages: 843-853
Abstract
Sulfolobus acidocaldarius a hyperthermoacidophilic archaeon possesses two βdecarboxylating dehydrogenase genes saci 0600 and saci 2375 in its genome which suggests that it uses these enzymes for three similar reactions in lysine biosynthesis through 2aminoadipate leucine biosynthesis and the tricarboxylic acid cycle To elucidate their roles these two genes were expressed in Escherichia coli in the present study and their gene products were characterized Saci 0600 recognized 3isopropylmalate as a substrate but exhibited slight and no activity for homoisocitrate and isocitrate respectively Saci 2375 exhibited distinct and similar activities for isocitrate and homoisocitrate but no detectable activity for 3isopropylmalate These results suggest that Saci 0600 is a 3isopropylmalate dehydrogenase for leucine biosynthesis and Saci 2375 is a dual function enzyme serving as isocitratehomoisocitrate dehydrogenase The crystal structure of Saci 0600 was determined as a closedform complex that binds 3isopropylmalate and Mg2+ thereby revealing the structural basis for the extreme thermostability and noveltype recognition of the 3isopropyl moiety of the substrateThis work was supported in part by JSPS KAKENHI Grant No 24228001 MN We are grateful to the staff of the Photon Factory for their assistance with data collection which was approved by the Photon Factory Program Advisory Committee Proposal nos 2011G524 2013G618 KL was supported by an ERC starting grant ARCHAELLUM 311523 and intramural funds from the Max Planck Society
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- Bio-removal of cadmium by growing deep-sea bacterium Pseudoalteromonas sp. SCSE709-6
- Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
- The amino acid composition of proteins from anaerobic halophilic bacteria of the order Halanaerobiales
- Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6
- The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii
- Structural and functional characterization of aspartate racemase from the acidothermophilic archaeon Picrophilus torridus
- Novel ultramicrobacterial isolates from a deep Greenland ice core represent a proposed new species, Chryseobacterium greenlandense sp. nov.
- Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity
- Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A
- The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
- Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world
- Archaeal diversity: temporal variation in the arsenic-rich creek sediments of Carnoulès Mine, France
- Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon
- Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan
- Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
- Identification of replication origins in the genome of the methanogenic archaeon, Methanocaldococcus jannaschii
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)
- Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia
- Identification of iron-reducing Thermus strains as Thermus scotoductus
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
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