Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: Jennifer LovelandCurtze Vanya Miteva Jean Brenchley
Publish Date: 2009/11/04
Volume: 14, Issue: 1, Pages: 61-69
Abstract
Three novel orange ultramicrobacterial isolates UMB10 UMB14 and UMB34T were isolated from enrichment cultures inoculated with a melted 3043 m deep Greenland ice core sample Phylogenetic analysis of the 16S rRNA gene sequences indicated that the isolates belonged to a single species within the genus Chryseobacterium They were most closely related to Chryseobacterium aquaticum 993 Chryseobacterium soli 971 and Chryseobacterium soldanellicola 969 Genomic hybridization showed low levels of relatedness between UMB34T and C aquaticum and C soldanellicola 19–30 and C soli and Chryseobacterium jejuense 45–56 Comparative genomic fingerprinting analysis using the enterobacterial repetitive intergenic consensus ERIC sequence showed nearly identical banding patterns for the three isolates and these patterns were distinct from those of C aquaticum C soldanellicola C soli and C jejuense The cells were short rods lacked flagella had cell volumes of 01 μm3 formed buds and smaller protrusions blebs produced copious extracellular material and a flexirubin type pigment UMB34T produced acids from carbohydrates and utilized glucose and maltose although it did not assimilate mannose The DNA G + C was 396–416 mol Based on the differences from validly named Chryseobacterium species it was concluded that these isolates represent a new species for which the name Chryseobacterium greenlandense is proposed The type strain is UMB34T =CIP 110007T = NRRL B59357This research was supported by National Science Foundation grant MO0347475 Department of Energy grant DEFG0293ER20117 and the National Aeronautics and Space Administration NASA Astrobiology Institute under NASA–Ames Cooperative Agreement NNA04CC06A We thank Missy Hazen for help with the electron microscopy work Dr Deborah Grove and Candace Price with DNA–DNA hybridization analysis and Alisa Inthavongsa with growth assays
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Other Papers In This Journal:
- Bio-removal of cadmium by growing deep-sea bacterium Pseudoalteromonas sp. SCSE709-6
- Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
- The amino acid composition of proteins from anaerobic halophilic bacteria of the order Halanaerobiales
- Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6
- The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii
- Structural and functional characterization of aspartate racemase from the acidothermophilic archaeon Picrophilus torridus
- Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity
- Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius
- Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A
- The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
- Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world
- Archaeal diversity: temporal variation in the arsenic-rich creek sediments of Carnoulès Mine, France
- Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon
- Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan
- Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
- Identification of replication origins in the genome of the methanogenic archaeon, Methanocaldococcus jannaschii
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)
- Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia
- Identification of iron-reducing Thermus strains as Thermus scotoductus
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
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