Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: T N R Srinivas S M Singh Suman Pradhan M S Pratibha K Hara Kishore Ashish K Singh Z Begum S R Prabagaran G S N Reddy S Shivaji
Publish Date: 2011/09/15
Volume: 15, Issue: 6, Pages: 673-
Abstract
Two 16S rRNA gene clone libraries KF and KS were constructed using two soil samples K7s and K8s collected near Kafni Glacier Himalayas The two libraries yielded a total of 648 clones Phyla Actinobacteria Bacteroidetes Chloroflexi Firmicutes Proteobacteria Spirochaetae Tenericutes and Verrucomicrobia were common to the two libraries Phyla Acidobacteria Chlamydiae and Nitrospirae were present only in KF library whereas Lentisphaerae and TM7 were detected only in KS In the two libraries clones belonging to phyla Bacteroidetes and Proteobacteria were the most predominant Principal component analysis PCA revealed that KF and KS were different and arsenic content influenced the differences in the percentage of OTUs PCA indicated that high water content in the K8s sample results in high total bacterial count PCA also indicated that bacterial diversity of KF and KS was similar to soils from the Pindari Glacier Himalayas Samoylov Island Siberia Schrimacher Oasis Antarctica and Siberian tundra The eleven bacterial strains isolated from the above two soil samples were phylogenetically related to six different genera All the isolates were psychro halo and alkalitolerant Amylase lipase and urease activities were detected in the majority of the strains Long chain saturated unsaturated and branched fatty acids were predominant in the psychrotolerant bacteriaWe would like to thank the Department of Biotechnology and Council of Scientific and Industrial Research Government of India for financial support to Dr S Shivaji TNRS acknowledges the CSIR Government of India for the award of research associateship
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