Journal Title
Title of Journal: Extremophiles
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Abbravation: Extremophiles
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Authors: Hanako Mochimaru Hideyoshi Yoshioka Hideyuki Tamaki Kohei Nakamura Nobuyuki Kaneko Susumu Sakata Hiroyuki Imachi Yuji Sekiguchi Hiroo Uchiyama Yoichi Kamagata
Publish Date: 2007/01/31
Volume: 11, Issue: 3, Pages: 453-461
Abstract
Microbial diversity and methanogenic potential in formation water samples from a dissolvedinwater type gas field were investigated by using 16S rRNA gene libraries and culturebased methods Two formation water samples of 46 and 53°C in temperature were obtained from a depth of 700 to 800 m Coenzyme F420autofluorescence indicated that 103–104 cells per ml of active methanogens were present accounting for at least 10 of the total cell count The 16S rRNA gene sequence analysis indicated that the diversity of Archaea and Bacteria of the two samples was quite limited ie the archaeal libraries were dominated by the sequences related to Methanobacterium formicicum and Methanothermobacter thermautotrophicus and the bacterial libraries were dominated by the sequences related to Hydrogenophilus and Deferribacter Of the methanogenic substrates tested using the formation waterbased medium only H2–CO2 gave rise to methane formation Those dominant archaeal and bacterial genera have the potential to use hydrogen for growth at the in situ temperatures suggesting that the formation water of the Pliocene strata in the gas field has been provided with hydrogen probably from underneath the strata and thus ongoing active methanogenesis has been occurring to dateWe gratefully acknowledge Yutaka Yamada of the Japan Energy Development Co Ltd for contributing the samples and for information about gas wells We are also grateful to Shuichi Tokuhashi of Advanced Industrial Science and Technology for helpful comments about the geological structure of the natural gas field in Japan
Keywords:
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- Bio-removal of cadmium by growing deep-sea bacterium Pseudoalteromonas sp. SCSE709-6
- Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
- The amino acid composition of proteins from anaerobic halophilic bacteria of the order Halanaerobiales
- Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6
- The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii
- Structural and functional characterization of aspartate racemase from the acidothermophilic archaeon Picrophilus torridus
- Novel ultramicrobacterial isolates from a deep Greenland ice core represent a proposed new species, Chryseobacterium greenlandense sp. nov.
- Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity
- Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius
- Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A
- The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
- Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world
- Archaeal diversity: temporal variation in the arsenic-rich creek sediments of Carnoulès Mine, France
- Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon
- Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
- Identification of replication origins in the genome of the methanogenic archaeon, Methanocaldococcus jannaschii
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Gene and primary structures of dye-linked l -proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
- Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)
- Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia
- Identification of iron-reducing Thermus strains as Thermus scotoductus
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
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