Authors: Fumitoshi Manabe Hirofumi Shoun Takayoshi Wakagi
Publish Date: 2011/04/02
Volume: 15, Issue: 3, Pages: 359-364
Abstract
A membraneintrinsic acid pyrophosphatase ST2226 from Sulfolobus tokodaii a thermoacidophilic archaeon is possibly involved in glycoprotein biosynthesis and belongs to the phosphatidic acid phosphatase class 2 superfamily including both membraneintrinsic and soluble enzymes with divergent functions ranging from dephosphorylation of undecaprenylpyrophosphate and phosphomonoesters such as glucose6phosphate to vanadiumcontaining chloroperoxidation ST2226 is an archaeal ortholog of these enzymes sharing a common phosphatase motif Through sitedirected mutagenesis as to each of the conserved residues the catalytic roles of the latter were deduced as well as the transmembrane topology with all the conserved residues in close proximity to the outside of the membrane
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