Osteogenic and adipogenic potential of porcine adi

Authors: Changqing Qu Guohua Zhang Lijie Zhang Gongshe Yang

Publish Date: 2007/02/17

Volume: 43, Issue: 2, Pages: 95-100

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Abstract

Human rat and mouse studies have demonstrated the existence of a population of adipose mesenchymal stem cells AMSCs that can undergo multilineage differentiation in vitro Understanding the clinical potential of AMSCs may require their use in preclinical largeanimal models such as pigs Thus the objectives of this study were to establish a protocol for the isolation of porcine AMSCs from adipose tissue and to examine their ex vivo differentiation potential to adipocytes and osteoblast The porcine AMSCs from passage 4 were selected for differentiation analysis The adipocytes were identified morphologically by staining with Oil Red O and the adipogenic marker genes were examined by RTPCR technique Osteogenic lineage was documented by deposition of calcium stained with Alzarin Red S visualization of alkaline phosphatase activity and expression of marker gene Our result indicates that porcine AMSCs have been successfully isolated and induced differentiation into adipocytes and osteoblasts This study suggested that porcine AMSCs are also a valuable model system for the study on the mesenchymal lineages for basic research and tissue engineeringThe authors are sincerely grateful to Dr Bin Wu for critically reading and revising the manuscript with English correction This work was supported in part by a grant of The State Key Basic Research and Development Plan of China 973 Program No 2004CB117506 and a grant 05ych017 of Innovation in Graduate Education Project funded by the Northwest AF University

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