Apigenin attenuates streptozotocininduced pancrea

Authors: Ning Wang Wen Jing Yi Lu Tan Jia Hui Zhang Jiamin Xu Yi Chen Mengting Qin Shuang Yu Jing Guan Rui Zhang

Publish Date: 2017/02/08

Volume: 53, Issue: 6, Pages: 554-563

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Abstract

Pancreatic beta cells are very sensitive to oxidative stress which is one of the major causes of cell damages in diabetes Growing interest has focused on the development of effective therapeutics to protect pancreatic cells from oxidative stress and searching for potentially protective antioxidants for treating diabetes Apigenin a plantderived flavonoid was investigated to determine whether it could protect rat insulinoma cell lines RINm5F pancreatic beta cells against streptozotocin STZinduced oxidative damages and the mechanisms implicated Our results showed that STZ treatment could induce oxidative stress and consequent cytotoxic effects in RINm5F cells Pretreatment with apigenin effectively decreased the intracellular reactive oxygen species ROS production attenuated cellular DNA damage diminished lipid peroxidation relieved protein carbonylation and restored the cell apoptosis of pancreatic beta cells stressed by STZ Our further experiments demonstrated that the beneficial effects of apigenin were related to ameliorate the loss of antioxidant enzymes of the STZtreated cells in the level of gene transcription protein expression and enzyme activity That suggested apigenin was not only a free radical scavenger but also a regulator to antioxidant defenses of pancreatic cells Taken all together our findings suggested that apigenin could attenuate the STZinduced oxidative damages in pancreatic beta cells and might serve as a novel agent for the treatment of diabetesThis study was supported by the National Natural Science Foundation of China Grant No 81300673 Startup Foundation for Advanced Talents of Jiangsu University Grant No 13JDG004 and Grant No 13JDG006 and Cultivation Project for Young Core Teacher of Jiangsu University

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