In vitro assessment of the combined effect of eico

Authors: Kamran A Mirza Menghua Luo Suzette Pereira Anne Voss Tapas Das Michael J Tisdale

Publish Date: 2016/05/18

Volume: 52, Issue: 8, Pages: 838-845

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Abstract

EPA has been clinically shown to reduce muscle wasting during cancer cachexia This study investigates whether curcumin or green tea extract GTE enhances the ability of low doses of eicosapentaenoic acid EPA to reduce loss of muscle protein in an in vitro model A low dose of EPA with minimal anticachectic activity was chosen to evaluate any potential synergistic effect with curcumin or GTE Depression of protein synthesis and increase in degradation was determined in C2C12 myotubes in response to tumour necrosis factorα TNFα and proteolysisinducing factor PIF EPA 50 μM or curcumin 10 μg ml−1 alone had little effect on protein degradation caused by PIF but the combination produced complete inhibition as did the combination with GTE 10 μg ml−1 In response to TNFα 25 ng ml−1induced protein degradation EPA had a small but not significant effect on protein degradation however when curcumin and GTE were combined with EPA the effect was enhanced EPA completely attenuated the depression of protein synthesis caused by TNFα but not that caused by PIF The combination of EPA with curcumin produced a significant increase in protein synthesis to both agents GTE alone or in combination with EPA had no effect on the depression of protein synthesis by TNFα but did significantly increase protein synthesis in PIFtreated cells Both TNFα and PIF significantly reduced myotube diameter from 17 to 13 μm for TNFα 235 and 15 μm 118 for PIF However the triple combination of EPA curcumin and GTE returned diameters to values not significantly different from the control These results suggest that either curcumin or GTE or the combination could enhance the anticatabolic effect of EPA on lean body massThis work was supported by a grant from Abbott Nutrition Columbus OH USA Kamran Ali Mirza and Michael J Tisdale declare that they have no conflict of interest Menghua Luo Suzette Pereira Anne C Voss and Tapas Das are employees of Abbott Nutrition

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