Responses of macrophages against Emphasis Type="I

Authors: Maozhi Hu Yun Yang Chuang Meng Zhiming Pan Xinan Jiao

Publish Date: 2013/08/16

Volume: 49, Issue: 10, Pages: 778-784

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Abstract

To explore the responses of host cell after infection with live Salmonella compared with phagocytosis to dead bacteria the responses of mouse macrophage after infection with Salmonella enteritidis C50041 and the fixed C50041 C50041d were analyzed Results indicated that the cytotoxicity induced by C50041 was stronger than C50041d Similar changing trends of mitochondrial membrane potential intracellular concentration of calcium ions reactive oxygen species and nitric oxide were found between C50041 and C50041d infection But the cell responses against C50041 were earlier and stronger than C50041d LC3 expression of macrophage induced by C50041 was lower than C50041d C50041 significantly inhibited the production of tumor necrosis factor and interleukin IL6 Whereas intracellular caspase1 activation and IL1β release induced by C50041 were stronger than C50041d caspase1 activation and IL1β release are the innate defense responses of macrophage Therefore it will be beneficial to explore the use of this pathway in the control of Salmonella infectionThis work was supported by the National Natural Science Foundation of China nos 31172299 and 31230070 the Program for New Century Excellent Talents in University NCET120745 “333” Program of Jiangsu Province BRA2011141 “333” “Six Talent Peaks Program” of Jiangsu Province NY028 “Qinglan Program” of Jiangsu Province 2012 the Program for Changjiang Scholars and Innovative Research Team in University IRT0978 the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Analysis and Test Research of Jiangsu New Technology and Standard

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