Role of calpastatin in the regulation of mRNA expr

Authors: Hoa Van Ba Bandugula Venkata Reddy Inho Hwang

Publish Date: 2014/12/09

Volume: 51, Issue: 5, Pages: 447-454

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Abstract

Calpastatin participates in apoptotic cell death and cell signaling but its role in skeletal myoblast development and molecular involvements in cell growth still remains unknown The current study aimed to investigate the role of calpastatin on the expression patterns of calpains caspases and heat shock proteins HSPs In addition the cell viability during myoblast growth under calpastatin silence condition was also investigated Three small interference RNA sequences siRNAs were used to silence calpastatin gene and ligated into pSilencer plasmid vector to construct short hairpin RNA shRNA expression The all three siRNAs significantly silence the calpastatin gene Moreover suppression of calpastatin significantly reduced the viability of myoblasts during growth phase when compared to control cells Additionally knockdown of calpastatin significantly increased the mRNA expression of μcalpain caspase3 caspase7 and caspase9 as well as HSP27 70 and 90 The present study results suggested that the suppression of calpastatin resulted in the increased expression of μcalpain caspases and HSPs which in turn regulate the apoptotic cell death The present study throws light on the central role of calpastatin in the control of calpain activity cell proliferation cell survival and apoptotic pathways

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