Effects of different omega3 sources fish oil kr

Authors: Kittisak Buddhachat Puntita Siengdee Siriwadee Chomdej Kumpanart Soontornvipart Korakot Nganvongpanit

Publish Date: 2017/01/11

Volume: 53, Issue: 5, Pages: 448-457

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Abstract

Our purpose was to evaluate the protective effect of three marine omega3 sources fish oil FO krill oil KO and greenlipped mussel GLM against cartilage degradation Canine cartilage explants were stimulated with either 10 ng/mL interleukin1β IL1β or IL1β/oncostatin M 10 ng/mL each and then treated with various concentrations of docosahexaenoic acid DHA eicosapentaenoic acid EPA 3 and 30 μg/mL FO KO or GLM 250 500 and 1000 μg/mL for 28 days Gene expression was then investigated in primary canine chondrocytes Our results showed that DHA and EPA as well as omega3 sources could suppress matrix degradation in cytokineinduced cartilage explants by significantly reducing the increase of sulfated glycosaminoglycans sGAGs and preserving uronic acid and hydroxyproline content except GLM These agents were not able to reduce IL1βinduced IL1B and TNFA expression but were able to downregulate the expression of the catabolic genes MMP1 MMP3 and MMP13 and upregulate the anabolic genes AGG and COL2A1 FO and KO were especially effective Our findings indicated that FO and KO were superior to GLM for their protective effect against proteoglycan and collagen degradation Hence FO and KO could serve as promising sources of chondroprotective agentsKN designed and conducted all the experiments KB assisted in the experiments performed the statistical analysis and gave supporting information for discussion KS administered some drugs and provided comments during the experiments KN KB PS and SC assisted in discussions and in writing of the manuscript All authors read and approved the final manuscript

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