Nestinpositive spheres derived from canine bone m

Authors: Hiroaki Kamishina Jennifer A Cheeseman Roger M Clemmons

Publish Date: 2008/04/09

Volume: 44, Issue: 5-6, Pages: 140-144

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Abstract

Bone marrow stromal cells BMSCs isolated from humans and rodents have been shown to generate neural cells under specific culture conditions and after transplantation in the central nervous system The apparent plasticity of BMSCs has therefore been a target of intensive research in attempt to develop a novel therapy for neurological diseases Canines sustain neurological disorders eg traumatic spinal cord injury that closely mirror pathology of those in humans Therefore we evaluated neural differentiation properties of canine BMSCs to provide insights into basic characterization of these cells for future neurotransplantation trials in canine patients with neurological disorders We demonstrate that canine BMSCs form spherical cellular aggregates on antiadhesive culture substrate in serumfree culture media which morphologically and phenotypically resemble spherical aggregates of neural progenitor cells socalled neurospheres Upon dissociation and subculture on adhesive substrate canine BMSCs express neuronal ß Шtubulin and glial GFAP A2B5 and CNPase markers Formation of spherical aggregates appears to be a critical preceding process for some of the glial marker expression CNPase and A2B5 However expression of more mature neuronal MAP2 and glial MBP markers could not be induced with the protocol used in this study We suggest that induction of canine BMSCs into cells with neural progenitor cell characteristics is possible and that these cells may have the potential for future cellular therapy for neurological disorders

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