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Title of Journal: Biol Plant

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Abbravation: Biologia Plantarum

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Publisher

Springer Netherlands

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DOI

10.1007/s003390101016

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ISSN

1573-8264

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Effect of growth regulators and ethylmethane sulph

Authors: A Junaid A Mujib M P Sharma
Publish Date: 2008/09/11
Volume: 52, Issue: 3, Pages: 569-572
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Abstract

A high efficient four step protocol callus initiation regeneration shoot elongation and rooting for in vitro propagation of Dracaena sanderiana Sander ex Mast was developed Callusing was achieved from nodal stem segment explants treated with various concentrations of ethylmethane sulphonate EMS on MS medium supplemented with 24dichlorophenoxyacetic acid 24D 15 g m−3 A significant increase in callus induction percentage and biomass production was noticed from lower EMS treated lines ET1 and ET2 comparatively to control and other ET3 ET4 and ET5 lines Calli of ET1 line showed high regeneration potential on MS medium with N 6benzylaminopurine BAP 175 g m−3 Length of microshoots which was reduced by EMS restored by addition of gibberellic acid GA3 04 g m−3 A marked increase in rooting with increasing EMS concentration was noticed on MS medium fortified with 3indolebutyric acid IBA 15 g m−3

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