Authors: ZB Qiu MM Yuan BZ Hai L Wang L Zhang
Publish Date: 2016/04/13
Volume: 60, Issue: 3, Pages: 427-434
Abstract
MicroRNAs miRNAs are small noncoding RNAs that play crucial regulatory roles in diverse developmental processes via cleavage or translational inhibition of their target mRNAs Although a growing number of miRNAs and their targets have been predicted and discovered via experimentation in many plants little is known about conserved miRNAs and their target genes in Pinus densata In the present study the conserved miRNAs miR171 and miR482 from Pinus densata were characterized Analysis of miR171 and miR482 reveal that these miRNAs were highly conserved in other plant species In addition the precursors of miR171 and miR482 were validated by real timePCR and sequencing Using realtime quantitative PCR miR171 and miR482 as well as their corresponding targets were found to be differentially expressed in needles stems and roots of Pinus densata Furthermore two target genes one GRAS family transcription factor protein gene and one nucleotidebinding site leucinerich repeat NBSLRR resistance protein gene were experimentally verified to be the targets of pdemiR171 and pdemiR482 respectively using RNA ligasemediated 5’rapid amplification of cDNA ends RLMRACE
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