Authors: C Fan Z Qiu B Zeng Y Liu X Li G Guo
Publish Date: 2016/11/14
Volume: 61, Issue: 3, Pages: 463-472
Abstract
Real time quantitative PCR qPCR is widely used in gene expression analysis for its accuracy and sensitivity Reference genes serving as endogenous controls are necessary for gene normalization In order to select an appropriate reference gene to normalize gene expression in Casuarina equisetifolia under salt stress 10 potential reference genes were evaluated using real time qPCR in the leaves and roots of plants grown under different NaCl concentrations and treatment durations GeNorm NormFinder and BestKeeper analyses reveal that elongation factor 1alpha EF1α and ubiquitinconjugating enzyme E2 UBC were the most appropriate reference genes for real time qPCR under salt stress However βtubulin βTUB and actin 7 which were widely used as reference genes in other plant species were not always stably expressed The combination of EF1α UBC uncharacterized protein 2 DNAJ homolog subfamily A member 2 and glyceraldehyde3phosphate dehydrogenase should be ideal reference genes for normalizing gene expression data in all samples under salt stress It indicates the need for reference gene selection for normalizing gene expression in C equisetifolia In addition the suitability of reference genes selected was confirmed by validating the expression of WRKY29like and expansinlike B1 The results enable analysis of salt response mechanism and gene expression in C equisetifolia
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