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Title of Journal: Biol Plant

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Abbravation: Biologia Plantarum

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Springer Netherlands

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DOI

10.1007/s12116-010-9072-x

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ISSN

1573-8264

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Expression of genes related to flavonoid and stilb

Authors: S Y Ahn S A Kim K S Cho H K Yun
Publish Date: 2014/07/05
Volume: 58, Issue: 4, Pages: 758-767
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Abstract

Recent studies have shown that the expression of genes related to the synthesis of flavonoids such as the phenylalanineammonia lyase PAL chalcone synthase CHS chalcone isomerase CHI and stilbene synthase STS genes were induced in response to different signaling molecules and Botrytis cinerea inoculation in grapevine leaves Therefore in the present study the nucleotide and deduced amino acid sequences of STS genes from cultivars Campbell Early and Kyoho were compared The deduced amino acid sequences of VlKSTS12 VlKSTS11 VICESTS13 and VlKSTS1 showed 100 homology to VlCESTS12 VlCESTS11 VICESTS24 and VlKSTS13 respectively in Campbell Early and Kyoho In addition the maximum transcription was observed 6 h after chemical treatments In Campbell Early the transcription of PAL CHS and CHI was higher in leaves treated with ethephon than in those treated with hydrogen peroxide methyl jasmonate and salicylic acid The PAL CHS and CHI genes were more induced in Campbell Early than in Kyoho The mRNA content of STS genes started to increase at 6 h and peaked at 48 h after the treatments In Kyoho leaves the expression of STSs was highly upregulated at 1 h and peaked at 6 h after the treatments The expression of the STS genes was induced in both the cultivars in leaves inoculated with B cinerea STS11 and STS12 showed differential expression patterns from STS1 STS24 and STS13 in Campbell Early leaves inoculated with B cinerea

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