Authors: H Sabouri A M Rezai A Moumeni A Kavousi M Katouzi A Sabouri
Publish Date: 2009/11/24
Volume: 53, Issue: 4, Pages: 657-662
Abstract
Oryza sativa L F2 population and F23 derived from a cross between salt tolerance cv Tarommahali and salt sensitive cv Khazar were used in this study A linkage map based on F2 population was constructed 74 SSR markers on 192 individuals which covered a total of 123150 cM with an average two locus interval of 1983 cM Two QTLs related to Na+/K+ ratio were found on chromosome 3 and 6 qDM3 and qDM8 for dry mass of shoot are major QTLs with very large effects explained 2090 and 1772 of the total phenotypic variance respectively Major locus for DM qDM3 was bracketed by RM1022 — RM6283 spread over 136 cM on chromosome 3 Major part of the variability for standard tolerance ranking STR was explained by the qSTR6 flanked by RM3727 — RM340 on chromosome 6 which exhibited phenotypic variance of 1725 and peak likelihood ratio LR of 1751 The length of this QTL is 88 cM and identification of any tightly linked markers in this region will serve as a candidate gene for finemapping qSTR3 overlapped with qNA3 and qNAK3 The qSTR3 may contain a new major gene for salt stress tolerance at seedling stage in rice Major QTLs identified in this paper after finemapping could be used for marker assisted selectionAcknowledgements This research was supported by Isfahan University of Technology and Rice Research Institute of Iran The authors are grateful to Ahmadreza Dadras and Ali Akbar Ebadi for their excellent technical assistance and greenhouse management
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