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Title of Journal: Biol Plant

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Abbravation: Biologia Plantarum

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Springer Netherlands

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DOI

10.1007/s10995-015-1727-9

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ISSN

1573-8264

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Activity of antioxidant enzymes and secondary meta

Authors: P Subhashini Devi B Satyanarayana A Arundhati T Raghava Rao
Publish Date: 2013/05/08
Volume: 57, Issue: 4, Pages: 778-782
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Abstract

The changes in the activities of antioxidant enzymes and amounts of proteins phenols and flavonoids in regenerating and nonregenerating calli during organogenesis of Sterculia urens were monitored Maximum growth of calli and the most efficient regeneration of shoots occurred on Murashige and Skoog MS medium supplemented with 05 mg dm−3 benzylaminopurine BAP + 2 or 4 mg dm−3 naphtalene acetic acid NAA Peroxidase POD catalase and superoxide dismutase activities increased in the regenerating calli but decreased in the nonregenerating calli Six POD isoenzymes were detected Protein content decreased in the nonregenerating calli and increased significantly during regeneration of shoots from callus Total phenols and flavonoids increased in the non regenerating calli SDSPAGE analysis revealed a role of many proteins in organogenesis

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