Authors: S Tereso C M Miguel M Mascarenhas A Roque H Trindade J Maroco M M Oliveira
Publish Date: 2008/09/11
Volume: 52, Issue: 3, Pages: 437-
Abstract
A highly reproducible system was developed for efficient rooting of cultivars Boa Casta BC and Peneda and a BC seedlingderived clone BC VII of almond Prunus dulcis Mill Twentyfour accessions derived from the clone BC VII and subjected to various in vitro culture treatments were screened The long induction pretreatment LIP 5 d the brief induction pretreatment BIP 16 h and the hormonal shock by short dipping in hormone solution 1 min were tested BIP was the only that allowed rooting of cultivars In BC VII it induced high rooting frequencies 47–100 when using a solution of 04 mM indole3butyric acid solidified with 2 g dm−3 gellam gum for 16h The response to the auxin type was variable depending on the cultivar and the root induction pretreatment used Root number was significantly different between the two cultivars and BC VII Root length was significantly higher when using 0005 mM IBA in LIP but this concentration induced apical necrosis The improved acclimatization procedure for up to 4 weeks increased the survival to 45 The initiation and development of adventitious roots were proved to be asynchronous
Keywords: