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Title of Journal: Folia Microbiol

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Abbravation: Folia Microbiologica

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Springer Netherlands

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DOI

10.1002/2015JG002993

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1874-9356

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Species identification of Emphasis Type="Italic"

Authors: R Kolínská M Dřevínek V Jakubů H Žemličková
Publish Date: 2008/12/16
Volume: 53, Issue: 5, Pages: 403-409
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Abstract

The Campylobacter species strains n = 42 isolated from clinical samples and deposited in Czech National Collection of Type Cultures Prague originally phenotypically and biochemically identified as Campylobacter jejuni were reclassified using molecular biological and mass spectrometric methods Wholecell MALDITOF MS matrixassisted laser desorption/ionization timeofflight mass spectrometry separated the isolates into two genetically related strains — C jejuni n = 26 and C coli n = 16 and moreover distinguished the intimate details in the group of tested strains It also made it possible to create the MALDITOF MS dendrogram similarly the spectral characteristics were used for the 3D cluster analysis Polymerase chain reaction PCR confirmed the results obtained by mass spectrometry Both methods PCR and MALDITOF MS gave the same results which supports their suitability in the rapid and accurate Campylobacterspecies determination

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