Journal Title
Title of Journal: Folia Microbiol
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Abbravation: Folia Microbiologica
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Publisher
Springer Netherlands
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Authors: O Tsypik O Yushchuk N Zaburannyi K Flärdh S Walker V Fedorenko B Ostash
Publish Date: 2015/10/03
Volume: 61, Issue: 3, Pages: 209-220
Abstract
Transcriptional factors of the GntR family regulate numerous physiological and morphological processes in response to the nutrient state of bacterial cells The number of GntR transcriptional factors in genomes of soildwelling actinomycetes is one of the highest among bacteria reflecting both the large size of their chromosomes and the complex ecological niche that they occupy However very little is known about the roles of GntRs in actinomycete biology Here we analyzed the genome of model actinomycete Streptomyces coelicolor A32 in an attempt to gain new insights into the function of GntR family All 56 GntR proteins of M145 strain were classified into FadR HutC MocR YtrA and DevA subfamilies according to their secondary structure We then checked for the presence of GntR orthologs in six other sequenced Streptomyces and one Kitasatospora genomes revealing that 12 GntRs were conserved in all analyzed strains Genomic analysis of the less studied YtrA type regulators revealed 160 sequences present in 88 members of Coriobacteridae Rubrobacteridae and Actinobacteridae subclasses These proteins form seven dense clusters on the consensus phylogenetic tree and their genes are usually colocated with the genes for transport proteins Probable operator sites were identified for orthologous groups of Sco0823 and Sco3812 proteins All S coelicolor YtrAlike regulatory genes SCO0823 SCO1728 SCO3812 were analyzed at transcriptional level knocked out and introduced on moderate copy number plasmid in M145 strain Also gene SCO0824 a part of putative SCO0823 operon was studied Results of these experiments are discussed hereThis work was supported by grants M/2562014 from the State Agency on Science Innovation and Informatization of Ukraine to BO and Bg98f from the Ministry of Education and Science of Ukraine VF Part of the project was carried out within the frame of the NIH grant R03TW009424 to SW and VF The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH Publication was partially based on the research provided by the grant support of the State Fund For Fundamental Research project № F60/22015 OT was supported by Visby and DAAD fellowships We thank Emma Doud Northwestern University USA for editing of our manuscript Prof Paul Dyson Swansea University UK is thanked for cosmids from Scoelicolor transposon library
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