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Title of Journal: Folia Microbiol

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Abbravation: Folia Microbiologica

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Springer Netherlands

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DOI

10.1007/bf00487930

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1874-9356

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Expression of fungal cellulase gene in Emphasis T

Authors: E Ozkose I Akyol B Kar U Comlekcioglu M S Ekinci
Publish Date: 2009/10/14
Volume: 54, Issue: 4, Pages: 335-342
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Abstract

The facultative anaerobic bacterium Lactococcus lactis has been used as a host for expression of a gene isolated from the anaerobic rumen fungus Neocallimastix sp The coding region of the cellulase gene was obtained from the fungus with the aid of polymerase chain reaction amplification The gene was then transformed into pCT vector system and the constructed recombinant plasmid was introduced into two L lactis strains IL403 and MG1363 by electroporation The gene encoding the fungal originated cellulase was expressed in both strains successfully although the expression level was relatively lower in comparison with the original enzyme activity Genetically modified L lactis strains were used as silage inoculants for prebiodegradation of the plant biomass during ensiling That treatment resulted in a notable reduction of the acid detergent fiber ADF and neutral detergent fiber NDF contents of the plant biomass used as silage material Inoculation with recombinant strain IL1043 resulted in 48 and 97 decrease in NDF and ADF contents respectively while the inoculation of silage with strain MG1363 decreased the ADF content by 5


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