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Title of Journal: Folia Microbiol

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Abbravation: Folia Microbiologica

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Springer Netherlands

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DOI

10.1007/bf00488952

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ISSN

1874-9356

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Phenotypic and genotypic characteristics of Empha

Authors: Osama Sammra Alice FriisMøller Anna Balbutskaya Muaz Hijazin Samy Nagib Jörg Alber Christoph Lämmler Amir Abdulmawjood Markus Timke Markus Kostrzewa Ellen PrengerBerninghoff
Publish Date: 2014/02/16
Volume: 59, Issue: 5, Pages: 369-374
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Abstract

Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically also including matrixassisted laser desorption ionization timeofflight mass spectrometry MALDITOF MS analysis and by genotypic methods The latter were performed by sequencing 16S rDNA and glyceraldehyde 3phosphate dehydrogenase encoding gene gap and by amplification of an A haemolyticum specific region of 16S–23S rDNA intergenic spacer region and 23S rDNA The six A haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin phospholipase D hemolysin A CAMP factor family protein collagen binding protein neuraminidase A and neuraminidase H which appeared to be present in two seven virulence genes two six virulence genes and two strains four virulence genes respectively The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A haemolyticum isolated from human patients a species which seems to be of increasing importance

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