Authors: Ewa Szczuka Katarzyna Urbańska Marta Pietryka Adam Kaznowski
Publish Date: 2012/06/19
Volume: 58, Issue: 1, Pages: 47-52
Abstract
Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms Therefore we investigated the biofilmforming ability of the methicillinresistant S aureus MRSA isolates collected from hospitalized patients As many as 96 strains had the ability to form biofilm in vitro The majority of S aureus strains formed biofilm in icadependent mechanism However 23 of MRSA isolates formed biofilm in icaindependent mechanism Half of these strains carried fnbB genes encoding surface proteins fibronectinbinding protein B involved in intercellular accumulation and biofilm development in S aureus strains The biofilm structures were examined via confocal laser scanning microscopy CLSM and threedimensional structures were reconstructed The images obtained in CLSM revealed that the biofilm created by icapositive strains was different from biofilm formed by icanegative strains The MRSA population showed a large genetic diversity and we did not find a single clone that occurred preferentially in hospital environment Our results demonstrated the variation in genes encoding adhesins for the host matrix proteins elastin laminin collagen fibronectin and fibrinogen and in the gene involved in biofilm formation icaA within the majority of S aureus clones
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