Authors: Qian Li Youhua Ren Guanzhou Qiu Nuo Li Hongwei Liu Zhimin Dai Xian Fu Li Shen Yili Liang Huaqun Yin Xueduan Liu
Publish Date: 2011/09/10
Volume: 56, Issue: 5, Pages: 439-
Abstract
To define the molecular response of Acidithiobacillus ferrooxidans under pH upshift temporal gene expression profiles were examined by using wholegenome DNA microarrays for A ferrooxidans Approximately 30 of the 3132 genes represented on the microarray were significantly upregulated over a 160min period while about 14 were significantly downregulated Our results revealed that A ferrooxidans showed potential selfprotection and selfregulation performance in response to pH upshift stress Many genes involved in regulation of membrane components were differentially expressed under the pH upshift stress Likewise most of genes involved in phosphate metabolism sulfur assimilation and CO2 fixation were obviously induced Conversely the transcription of a polyphosphate kinase gene AFE1210 associated with phosphate storage was significantly repressed which probably stemmed from the depletion of polyphosphate Besides most of the genes involved in hydrogen uptake were significantly induced whereas many genes involved in nitrogen fixation were obviously repressed which suggested that hydrogen uptake and nitrogen fixation could contribute to cytoplasmic pH homeostasisThis research was supported by the National Natural Science Foundation of China no 31070104 30770051 and the National Basic Research Program no 2010CB630901 Doctoral Fund of Ministry of Education of China no 200805330035 and New Teacher Fund for Doctor Station the Ministry of Education no 200805331078
Keywords: