Journal Title
Title of Journal: Folia Microbiol
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Abbravation: Folia Microbiologica
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Publisher
Springer Netherlands
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Authors: M Marejková H Roháčová M Reisingerová P Petráš
Publish Date: 2012/01/19
Volume: 57, Issue: 2, Pages: 85-89
Abstract
A large outbreak caused by a rare Shiga toxinproducing Escherichia coli serotype O104H4 occurred in Germany in May to July 2011 The National Reference Laboratory for E coli and Shigella investigated the stool sample from an American tourist with bloody diarrhea who arrived in the Czech Republic from Germany where she consumed salads with raw vegetable a week ago Using culture of the enriched stool on extendedspectrum βlactamase agar we isolated E coli strain which belonged to serotype O104H4 as determined by conventional and molecular serotyping The strain contained the major virulence characteristics of enterohemorrhagic E coli stx 2 encoding Shiga toxin 2 and enteroaggregative E coli aggA encoding aggregative adherence fimbriae I This unique combination of virulence traits demonstrated that this strain belongs to the hybrid enteroaggregative hemorrhagic E coli clone which caused the German outbreak Using advanced culture and molecular biological approaches is the prerequisite for identification of new unusual pathogensThe first author is indebted to Prof Dr H Karch the Head of the National Consulting Laboratory for Hemolytic–Uremic Syndrome at the Institute for Hygiene University of Münster Germany for the possibility to get trained in this laboratory in diagnosis of EHEC infections The authors are also thankful to Assoc Prof Dr M Bielaszewska from the above Institute for assistance provided to the Czech NRL for E coli and Shigella especially during the hectic time of the German outbreak and for discussions during the preparation of this manuscript The authors thank the laboratory colleagues in particular Ms M Vašáková and the collaborating microbiologists and clinicians for providing the patients’ data The authors also thank BioVendor Ltd Czech Republic for longterm access to the device Microflex for MALDITOF MS identification of bacteria
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