Authors: Yoshihide Miura Kaoru Kanazawa Noriko Yokoo Kazue Iizawa Masayuki Okada Shinya Oda Masaki Nakane
Publish Date: 2010/02/18
Volume: 24, Issue: 2, Pages: 234-239
Abstract
Prostaglandin E1 PGE1 has been shown to provide shortterm neuroprotection against various types of brain ischemia in a dosedependent manner in mice However these findings were obtained from experiments performed without any control over physiological parameters We performed an outcome study where physiological parameters were controlled in an attempt to confirm the dosedependant neuroprotective effects of PGE1A rat model of severe forebrain ischemia was used Two doses of PGE1 were administered during the preischemic period a low dose LowPG group and a high dose HighPG group Normotension was maintained in the LowPG group while hypotension was induced in the HighPG group In separate groups normal saline Control or sodium nitroprusside SNP were infused to compare outcomes under similar blood pressure conditions Histological outcomes in the hippocampal CA1 and entorhinal cortex were evaluated 5 days postischemiaHighPG resulted in hyperglycemia The percentage of dead neurons in the hippocampal CA1 and entorhinal cortex were similar in the Control SNP and HighPG groups the percentage being significantly attenuated in the LowPG group CA1 Control = 928 ± 24 LowPG = 850 ± 85 HighPG = 953 ± 24 and SNP = 964 ± 07 P 001 entorhinal cortex Control = 738 ± 40 LowPG = 532 ± 123 HighPG = 721 ± 126 and SNP = 765 ± 41 P 001This work was supported by a research fund of the Department of Anesthesiology Yamagata University School of Medicine and by Ono Pharmaceutical Co Ltd Osaka Japan Ono Pharmaceutical Co kindly provided free samples of PGE1 prostandin The authors are grateful to the late Professor Emeritus Hideo Horikawa for his instruction in performing the study We also wish to thank Mr Tadayoshi Karube and Mrs Michiko Sakai for their expert technical assistance
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